About fit libraries

Fit libraries are lists of possible positions of your input structures in the EM map. The positions are used in the global optimization step to generate a large number of combinations of the fits through a Monte Carlo integrative modeling procedure. Each position has its associated score and p-value, which are used as EM restraints during the modeling.

Read more about fit libraries in the manual.

The fit libraries are typically generated by fitting every input structure into the EM map separately. For this tutorial, the fit libraries are already provided in the fits/ directory.

Follow the next steps if you want to generate them yourself using Efitter package, which is a wrapper around UCSF Chimera FitMapTool .

To skip and jump to integrative modeling, move to Setting up the JSON project file.