About the Elongator complex
Elongator is a complex involved in tRNA modification. In yeast, it contains six subunits, two copies each.
We built and published an integrative model of yeast Elongator in 2017. The cryo-EM structure published in 2019 confirmed the model.
In this tutorial, we will model a subcomplex of Elongator composed of subunits named Elp1, Elp2, and Elp3, using the real data from the 2017 work.
The following PDB files are available for the subunits:
The following experimental data will be used as restraints:
a negative stain EM map at 27 Å (EMD-4151):
The map exhibits a C2 symmetry, thus the model will be built with C2 symmetry.
crosslinks from a crosslinking mass spectrometry experiment (details here)