Tutorial: integrative modeling of Elongator complex using Assembline

Assembline is a software for integrative structural modeling of macromolecular assemblies - an assembly line of macromolecular assemblies!

This tutorial demonstrates a basic usage of Assembline on an example of Elongator complex.

Use the Next button at the bottom right or menu on the left to navigate through the tutorial.

• About the Elongator complex

Set up

• Install Assembline

Data preparation

• Data preparation

Calculation of fit libraries

• About fit libraries
• Set up
• Run
• Analyze fits
  • Check if run correctly

Global optimization

• Setting up the JSON project file
  • Create Xlink Analyzer project
  • Add modeling information to the project file
• Setting up the parameter file
• Run
• Analyze
  • Extract scores
  • Create a CIF file of the top 10 models
  • Quick checks
  • Assess sampling exhaustiveness
  • Conclusions

Refinement

• Setting up the JSON project file
  • Generating the file from scratch
• Setting up the parameter file
• Run
• Analyze
  • Extract scores
  • Create a CIF file of the top 10 models
  • Quick convergence check:
  • Assess sampling exhaustiveness
  • Conclusions

TO DO LIST